BC Centre for Excellence in HIV/AIDS View Institution's Website 2 articles published in JoVE Application Notes Inferencia Genotipica de tropismo VIH-1 usando secuanciacion de V3 basada en poblacion - ADVERTISEMENT Rachel A. McGovern1, P. Richard Harrigan1, Luke C. Swenson1 1Laboratory Program, BC Centre for Excellence in HIV/AIDS Immunology and Infection Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3 Rachel A. McGovern1, P. Richard Harrigan1, Luke C. Swenson1 1Laboratory Program, BC Centre for Excellence in HIV/AIDS HIV tropism can be inferred from the V3 region of the viral envelope. V3 is PCR amplified in triplicate using nested RT-PCR, sequenced, and interpreted using bioinformatic software. Samples with with 1 or more sequence(s) with low g2P scores are classified as non-R5 virus.
