2 articles published in JoVE
Nerve-sparing Mid-urethral Obstruction (NeMO) in Female Small Rodents Martin Sidler1,2,3, Karen J. Aitken1, Jia Xin Jiang4, Darius J. Bägli1,2,3 1Developmental and Stem Cell Biology, Research Institute, The Hospital for Sick Children, 2Institute of Medical Science, University of Toronto, 3Pediatric Urology, The Hospital for Sick Children, 4Molecular Structure and Function, Research Institute, The Hospital for Sick Children Traditional modeling of partial bladder outlet obstruction in rodents is fraught with animal mortality. A denervation injury from dissection around the proximal urethra and bladder neck is also of major concern. We developed and evaluated a safe and reliable mid-urethral obstruction model, avoiding the shortcomings of the traditional model.
Multiple-mouse Neuroanatomical Magnetic Resonance Imaging Jun Dazai*1, Shoshana Spring*1, Lindsay S. Cahill1, R. Mark Henkelman1,2 1Mouse Imaging Centre, Hospital for Sick Children, 2Department of Medical Biophysics and Medical Imaging, University of Toronto Magnetic resonance imaging (MRI) has become an increasingly popular tool for examining the phenotype of genetically altered mice. This article illustrates the methods necessary to achieve high-throughput phenotyping of genetically altered mice using multiple-mouse MRI.