3 articles published in JoVE
Composition and Properties of Aquafaba: Water Recovered from Commercially Canned Chickpeas Youn Young Shim1,2,3, Rana Mustafa1, Jianheng Shen1, Kornsulee Ratanapariyanuch1, Martin J. T. Reaney1,2,3 1Department of Plant Sciences, University of Saskatchewan, 2Prairie Tide Chemicals Inc., 3Guangdong Saskatchewan Oilseed Joint Laboratory (GUSTO), Department of Food Science and Engineering, Jinan University Aquafaba is a viscous juice from canned chickpea that, when stirred vigorously, produces a relatively stable white froth or foam. The primary research goal is to identify the components of aquafaba that contribute viscosifying/thickening properties using nuclear magnetic resonance (NMR), ultrafiltration, electrophoresis, and peptide mass fingerprinting.
Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres Tim J. Dumonceaux1, Jennifer R. Town1, Janet E. Hill2, Bonnie L. Chaban2, Sean M. Hemmingsen3 1Saskatoon Research Centre, Agriculture and Agri-Food Canada, 2Department of Veterinary Microbiology, University of Saskatchewan, 3Plant Biotechnology Institute, National Research Council of Canada We describe a multiplex method for the detection of microorganisms within a sample using oligonucleotide-coupled fluorescent beads. Amplicon from all organisms within a sample is hybridized to a panel of probe-coupled beads. A Luminex or Bio-Plex instrument is used to query each bead for bead type and hybridization signal.
Tracking Neutrophil Intraluminal Crawling, Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy Najia Xu1, Xi Lei1, Lixin Liu1 1Department of Pharmacology, University of Saskatchewan We describe a protocol of brightfield intravital microscopy for measuring dynamic neutrophil-endothelial cell interactions during neutrophil recruitment in response to the source of a neutrophil chemoattractant in vivo. Neutrophil intraluminal crawling, transendothelial migration and chemotaxis in mouse cremaster muscle tissue are visualized with time-lapsed video photography and tracked with ImageJ.