3 articles published in JoVE
Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands Elham Dianati1,2, Isabelle Plante1 1INRS, Institut Armand-Frappier, 2Biomed, UQAM Intercellular junctions are requisites for mammary gland stage-specific functions and development. This manuscript provides a detailed protocol for the study of protein-protein interactions (PPIs) and co-localization using murine mammary glands. These techniques allow for the investigation of the dynamics of the physical association between intercellular junctions at different developmental stages.
Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption Lucas Sagrillo-Fagundes*1, Hélène Clabault*1, Laetitia Laurent*1, Andrée-Anne Hudon-Thibeault1, Eugênia Maria Assunção Salustiano1, Marlène Fortier1, Josianne Bienvenue-Pariseault1, Philippe Wong Yen1, J. Thomas Sanderson1, Cathy Vaillancourt1 1INRS-Institut Armand-Frappier This manuscript presents a unique in vitro model of immunopurified human villous cytotrophoblast cells cultured under hypoxia/reoxygenation. This model is suitable to study the protective effects of promising treatments, such as melatonin, on pregnancy complications associated with increased oxidative stress and altered placental function.
Preparation, Imaging, and Quantification of Bacterial Surface Motility Assays Nydia Morales-Soto1,2, Morgen E. Anyan1, Anne E. Mattingly1, Chinedu S. Madukoma1, Cameron W. Harvey3, Mark Alber3, Eric Déziel4, Daniel B. Kearns5, Joshua D. Shrout1,2,6 1Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, 2Eck Institute for Global Health, University of Notre Dame, 3Department of Applied and Computational Mathematics and Statistics, University of Notre Dame, 4INRS-Institut Armand-Frappier, 5Department of Biology, Indiana University, 6Department of Biological Sciences, University of Notre Dame Swarming motility is influenced by physical and environmental factors. We describe a two-phase protocol and guidelines to circumvent the challenges commonly associated with swarm assay preparation and data collection. A macroscopic imaging technique is employed to obtain detailed information on swarm behavior that is not provided by current analysis techniques.