Universite du Quebec a Montreal 5 articles published in JoVE Genetics Measuring Microbial Mutation Rates with the Fluctuation Assay Rok Krašovec1, Huw Richards2,3, Guillaume Gomez3, Danna R. Gifford1, Adrien Mazoyer4, Christopher G. Knight3 1School of Biological Sciences, The University of Manchester, 2School of Science, University of Waikato, 3School of Natural Sciences, The University of Manchester, 4Department of Mathematics, Université du Québec à Montréal Here, a protocol is presented to perform a fluctuation assay and estimate microbial mutation rate using phenotypic markers. This protocol will enable researchers to assay mutations in diverse microbes and environments, determining how genotype and ecological context affect spontaneous mutation rates. Immunology and Infection Processing of Bronchoalveolar Lavage Fluid and Matched Blood for Alveolar Macrophage and CD4+ T-cell Immunophenotyping and HIV Reservoir Assessment Syim Salahuddin*1,2, Elaine Thomson*1,2,3, Oussama Méziane1,2, Omar Farnos2, Amélie Pagliuzza5, Nicolas Chomont5,6, Ron Olivenstein1, Cecilia Costiniuk1,3,4, Mohammad-Ali Jenabian2,3,6 1Research Institute McGill University Health Centre, 2Department of Biological Sciences, Université de Québec à Montréal, 3Department of Microbiology & Immunology, McGill University, 4Department of Medicine, Division of Infectious Diseases, McGill University, 5 We describe a method for processing bronchoalveolar lavage fluid and matched peripheral blood from chronically HIV-infected individuals on antiretroviral therapy to assess pulmonary HIV reservoirs. These methods result in the acquisition of highly pure CD4 T cells and alveolar macrophages that may subsequently be used for immunophenotyping and HIV DNA/RNA quantifications by ultrasensitive polymerase chain reaction. Developmental Biology Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands Elham Dianati1,2, Isabelle Plante1 1INRS, Institut Armand-Frappier, 2Biomed, UQAM Intercellular junctions are requisites for mammary gland stage-specific functions and development. This manuscript provides a detailed protocol for the study of protein-protein interactions (PPIs) and co-localization using murine mammary glands. These techniques allow for the investigation of the dynamics of the physical association between intercellular junctions at different developmental stages. Neuroscience Novel Metrics to Characterize Embryonic Elongation of the Nematode Caenorhabditis elegans Emmanuel Martin1, Olivier Rocheleau-Leclair1, Sarah Jenna1 1Department of Chemistry, Pharmaqam, Université du Québec à Montréal Here we detail protocols specifically designed to monitor morphogenic defects that occur during early and late phases of embryonic elongation of the nematode Caenorhabditis elegans. Ultimately, these protocols are designed to identify genes that regulate these phases and to characterize their differential requirements along the antero-posterior axis of the embryo. Neuroscience A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors Karl-Frederik Bergeron1, Tatiana Cardinal1, Nicolas Pilon1 1Molecular Genetics of Development Laboratory, Biological Sciences, UQAM We present an ex vivo cell migration assay that allows precise quantification of enteric neural crest cell migration potential in the presence of various growth factors.
