3 articles published in JoVE
Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands Elham Dianati1,2, Isabelle Plante1 1INRS, Institut Armand-Frappier, 2Biomed, UQAM Intercellular junctions are requisites for mammary gland stage-specific functions and development. This manuscript provides a detailed protocol for the study of protein-protein interactions (PPIs) and co-localization using murine mammary glands. These techniques allow for the investigation of the dynamics of the physical association between intercellular junctions at different developmental stages.
Novel Metrics to Characterize Embryonic Elongation of the Nematode Caenorhabditis elegans Emmanuel Martin1, Olivier Rocheleau-Leclair1, Sarah Jenna1 1Department of Chemistry, Pharmaqam, Université du Québec à Montréal Here we detail protocols specifically designed to monitor morphogenic defects that occur during early and late phases of embryonic elongation of the nematode Caenorhabditis elegans. Ultimately, these protocols are designed to identify genes that regulate these phases and to characterize their differential requirements along the antero-posterior axis of the embryo.
A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors Karl-Frederik Bergeron1, Tatiana Cardinal1, Nicolas Pilon1 1Molecular Genetics of Development Laboratory, Biological Sciences, UQAM We present an ex vivo cell migration assay that allows precise quantification of enteric neural crest cell migration potential in the presence of various growth factors.