3 articles published in JoVE
High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping Ceres Kosuta1,2, Kate Daniel1, Devon L. Johnstone1,2, Kevin Mongeon1,2, Kevin Ban1,2, Sophie LeBlanc1, Stuart MacLeod1, Karim Et-Tahiry2, Marc Ekker2, Alex MacKenzie1, Izabella Pena1,2 1 Zebrafish have been used as reliable genetic model organisms in biomedical research, especially with the advent of gene-editing technologies. When larval phenotypes are expected, DNA extraction and genotype identification can be challenging. Here, we describe an efficient genotyping procedure for zebrafish larvae, by tail clipping, as early as 72-h post-fertilization.
Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy Kristina J. Allan1,2, Douglas J. Mahoney1,4, Stephen D. Baird1, Charles A. Lefebvre1, David F. Stojdl1,2,3 1 Here we describe a protocol for employing high-throughput RNAi screening to uncover host targets that can be manipulated to enhance oncolytic virus therapy, specifically rhabodvirus and vaccinia virus therapy, but it can be readily adapted to other oncolytic virus platforms or for discovering host genes that modulate virus replication generally.
Isolation of CD146+ Resident Lung Mesenchymal Stromal Cells from Rat Lungs Jennifer J.P. Collins1,2, Marius A. Möbius1,3,4, Bernard Thébaud1,2,5 1Sinclair Centre for Regenerative Medicine, Ottawa Hospital Research Institute, 2University of Ottawa, 3Department of Neonatology and Pediatric Critical Care Medicine, Medical Faculty and University Hospital Carl Gustav Carus, Technische Universität Dresden, 4DFG Research Center and Cluster of Excellence for Regenerative Therapies (CRTD), Technische Universität, Dresden, 5 This protocol describes an isolation technique for obtaining primary lung resident mesenchymal stromal cells from rats, through the use of enzymatic digestion, density gradient separation, plastic adherence and CD146+ magnetic bead selection.