3 articles published in JoVE
Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases Ryan J. Mailloux1, Adrian Young1, Marisa O'Brien1, Robert Morris Gill1 1Department of Biochemistry, Memorial University of Newfoundland Mitochondria contain several flavin-dependent enzymes that can produce reactive oxygen species (ROS). Monitoring ROS release from individual sites in mitochondria is challenging due to unwanted side reactions. We present an easy, inexpensive method for direct assessment of native rates for ROS release using purified flavoenzymes and microplate fluorometry.
Paw-Dragging: a Novel, Sensitive Analysis of the Mouse Cylinder Test R. Brian Roome1,2, Jacqueline L. Vanderluit1 1BioMedical Sciences, Faculty of Medicine, Memorial University of Newfoundland, 2Neurosciences, Faculty of Medicine, McGill University Classical forelimb asymmetry analysis of the cylinder test is routinely used to assess behavioural deficits in rats following brain injury or stroke; however, it fails to detect consistent deficits in mice. This study demonstrates that quantifying paw-dragging behaviour is a more sensitive analysis of brain injury in mice.
A Lateralized Odor Learning Model in Neonatal Rats for Dissecting Neural Circuitry Underpinning Memory Formation Christine J. Fontaine*1,2, Bandhan Mukherjee*1, Gillian L. Morrison1, Qi Yuan1 1Division of Biomedical Sciences, Faculty of Medicine, Memorial University, 2Division of Medical Sciences, University of Victoria This protocol introduces lateralized early odor preference learning in rats using acute single naris occlusion. Lateralized learning permits the examination of behavioral outcomes and underpinning biological mechanisms within the same animals, reducing variance induced by between-animal designs. This protocol can be used to investigate molecular mechanisms underpinning early odor learning.