CHU de Quebec Research Center 4 articles published in JoVE Medicine Three-Dimensional Culture Assay to Explore Cancer Cell Invasiveness and Satellite Tumor Formation Marie-France Côté1, Audrey Turcotte1,2, Charles Doillon1,3, Stephane Gobeil1,2 1CHU de Québec Research Centre, 2Department of Molecular Medicine, Laval University, 3Department of Surgery, Laval University Cancer cells are embedded in a collagen gel and then sandwiched in an acellular fibrin gel to generate a 3D culture system in which the invasiveness and formation of satellite tumors may be monitored. Neuroscience Correlative Light and Electron Microscopy to Study Microglial Interactions with β-Amyloid Plaques Kanchan Bisht*1, Hassan El Hajj*1, Julie C. Savage1, Maria G. Sánchez1, Marie-Ève Tremblay1 1Neurosciences Axis, CHU de Québec Research Center This article describes a protocol for visualizing amyloid Aβ plaques in Alzheimer's disease mouse models using methoxy-X04, which crosses the blood-brain barrier and selectively binds to β-pleated sheets found in dense core Aβ plaques. It allows pre-screening of plaque-containing tissue sections prior to immunostaining and processing for electron microscopy. Developmental Biology Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures Julie Lessard1, Julie Anne Côté1, Marc Lapointe1, Mélissa Pelletier2, Mélanie Nadeau1, Simon Marceau1, Laurent Biertho1, André Tchernof1,2,3 1IUCPQ Research Center, 2CHU de Québec Research Center, 3Laval University Mature adipocytes may represent an abundant source of stem cells through dedifferentiation, which leads to a homogenous population of fibroblast-like cells. Collagenase digestion is used to isolate mature adipocytes from human fat. The goal of our protocol is to obtain multipotent, dedifferentiated fat cells from human mature adipocytes. Biology Analysis of Translation Initiation During Stress Conditions by Polysome Profiling Laëtitia Coudert*1,2, Pauline Adjibade*1,2, Rachid Mazroui1,2 1Department of Molecular Biology, Medical Biochemistry, and Pathology, Faculty of Medicine, Laval University, 2CHU de Quebec Research Center Here, we describe a method to analyze changes in the initiation of mRNA translation of eukaryotic cells in response to stress conditions. This method is based on the velocity separation on sucrose gradients of translating ribosomes from non-translating ribosomes.
