3 articles published in JoVE
siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts Adjimon Gatien Lokossou1, Chirine Toufaily2, Amandine Vargas3, Benoit Barbeau1 1Department of Biological Sciences, University of Quebec in Montreal, 2Department of Pharmacology and Therapeutics, McGill University, 3Department of Clinical Sciences, University of Montreal This protocol describes a method for efficiently transfecting siRNA in freshly isolated human villous cytotrophoblasts using microporation and identifying DNA-protein complexes in these cells. Transfected cells can be monitored by Western blot and EMSA analyses during the 4-day culture time.
Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact Justine Renaud1, Maria-Grazia Martinoli1 1Dept. of Medical Biology, University of Québec In multicellular organisms, secreted soluble factors elicit responses from different cell types as a result of paracrine signaling. Insert co-culture systems offer a simple way to assess the changes mediated by secreted soluble factors in the absence of cell-cell contact.
Assessing the Multiple Dimensions of Engagement to Characterize Learning: A Neurophysiological Perspective Patrick Charland1, Pierre-Majorique Léger2, Sylvain Sénécal3, François Courtemanche2, Julien Mercier4, Yannick Skelling1, Elise Labonté-Lemoyne2 1Department of Didactics, Université du Québec à Montréal, 2Department of IT and Tech3Lab, HEC Montreal, 3Department of Marketing and Tech3Lab, HEC Montreal, 4Department of Specialized Education, Université du Québec à Montréal This paper aims to describe the techniques involved in the collection and synchronization of the multiple dimensions (behavioral, affective and cognitive) of learners’ engagement during a task.