Canadian Institute for Advanced Research

2 articles published in JoVE

• Immunology and Infection

  
  Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging Katharine M. Ng^1,2, Carolina Tropini^{1,2,3 1}School of Biomedical Engineering, University of British Columbia, ²Department of Microbiology and Immunology, University of British Columbia, ³Humans and the Microbiome Program, Canadian Institute for Advanced Research (CIFAR) This streamlined protocol details a workflow to detect and image bacteria in complex tissue samples, from fixing the tissue to staining microbes with fluorescent in situ hybridization.

• Neuroscience

  
  Foraging Path-length Protocol for Drosophila melanogaster Larvae Ina Anreiter¹, Oscar E. Vasquez¹, Aaron M. Allen², Marla B. Sokolowski^{1,3 1}Department of Ecology and Evolutionary Biology, University of Toronto, ²Department of Cell and Systems Biology, University of Toronto, ³Child and Brain Development Program, Canadian Institute for Advanced Research We provide a detailed protocol for a Drosophila melanogaster foraging path-length assay. We discuss the preparation and handling of test animals, how to perform the assay and analyze the data.