Vancouver Costal Health Research Institute 1 article published in JoVE Biology Transfecting RAW264.7 Cells with a Luciferase Reporter Gene Sylvia T. Cheung1,2,3, Soroush Shakibakho1,2, Eva Y. So1,2, Alice L-F Mui1,2,3 1Immunity and Infection Research Centre, Vancouver Costal Health Research Institute, 2Department of Surgery, University of British Columbia, 3Department of Biochemistry and Molecular Biology, University of British Columbia Transfection into the macrophage cell line, RAW264.7, is difficult due to the cell’s natural response against foreign materials. We described here a gentle yet robust procedure for transfecting luciferase reporter genes into RAW264.7 cells.
