Health Sciences Centre 1 article published in JoVE Neuroscience Low-Density Primary Hippocampal Neuron Culture Reiko T. Roppongi*1,2, Kevin P. Champagne-Jorgensen*1,2, Tabrez J. Siddiqui1,2 1Department of Physiology and Pathophysiology, University of Manitoba, 2Kleysen Institute for Advanced Medicine, Health Sciences Centre This article describes the protocol for culturing low-density primary hippocampal neurons growing on glass coverslips inverted over a glial monolayer. The neuron and glial layers are separated by paraffin wax beads. The neurons grown by this method are suitable for high-resolution optical imaging and functional assays.
