Hopital Maisonneuve-Rosemont 2 articles published in JoVE Biology Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry Benoîte Bourdin1, Emilie Segura1, Marie-Philippe Tétreault1, Sylvie Lesage2, Lucie Parent1 1Département de Physiologie Moléculaire et Intégrative, Montreal Heart Institute Research Centre, 2Département de Microbiologie, Infectiologie, Immunologie, Centre de recherche de l'Hôpital Maisonneuve-Rosemont Inherited cardiac arrhythmias are often caused by mutations that alter the surface delivery of one or more ion channels. Here, we adapt flow cytometry assays to provide a quantification of the relative total and cell surface protein expression of recombinant ion channels expressed in tsA-201 cells. Immunology and Infection Preparation and Applications of Organotypic Thymic Slice Cultures Aditi Sood*1,2, Mengqi Dong*1,2, Heather J. Melichar1,3 1Centre de Recherche, Hôpital Maisonneuve-Rosemont, 2Department of Microbiology, Infectiology and Immunology, Université de Montréal, 3Department of Medicine, Université de Montréal We describe the preparation of thymic slices that, in combination with flow cytometry, can be used to model positive and negative selection of developing T cells. Thymic slices can also be adapted for the in situ analysis of thymocyte migration, localization, and signaling via immunofluorescence and two-photon microscopy.
