2 articles published in JoVE
A Magnetic Microbead Occlusion Model to Induce Ocular Hypertension-Dependent Glaucoma in Mice Yoko A. Ito*1, Nicolas Belforte*1, Jorge L. Cueva Vargas1, Adriana Di Polo1 1Department of Neuroscience, CRCHUM and University of Montreal Here, we present a protocol to induce ocular hypertension in the murine eye that results in the loss of retinal ganglion cells as observed in glaucoma. Magnetic microbeads are injected into the anterior chamber and attracted to the iridocorneal angle using a magnet to block the outflow of aqueous humour.
Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria Sarah Pickles1, Nathalie Arbour2, Christine Vande Velde2 1Department of Biochemistry, Université de Montréal, CRCHUM, 2Department of Neurosciences, Université de Montréal, CRCHUM Described here is a method to detect and quantify mitochondrial outer membrane proteins by immunolabeling of mitochondria isolated from rodent tissue and analysis by flow cytometry. This method can be extended to assess functional aspects of mitochondrial subpopulations.