CRCHUM and University of Montreal 3 articles published in JoVE Medicine A Magnetic Microbead Occlusion Model to Induce Ocular Hypertension-Dependent Glaucoma in Mice Yoko A. Ito*1, Nicolas Belforte*1, Jorge L. Cueva Vargas1, Adriana Di Polo1 1Department of Neuroscience, CRCHUM and University of Montreal Here, we present a protocol to induce ocular hypertension in the murine eye that results in the loss of retinal ganglion cells as observed in glaucoma. Magnetic microbeads are injected into the anterior chamber and attracted to the iridocorneal angle using a magnet to block the outflow of aqueous humour. Biology Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria Sarah Pickles1, Nathalie Arbour2, Christine Vande Velde2 1Department of Biochemistry, Université de Montréal, CRCHUM, 2Department of Neurosciences, Université de Montréal, CRCHUM Described here is a method to detect and quantify mitochondrial outer membrane proteins by immunolabeling of mitochondria isolated from rodent tissue and analysis by flow cytometry. This method can be extended to assess functional aspects of mitochondrial subpopulations. Medicine A Model of Chronic Nutrient Infusion in the Rat Grace Fergusson1, Mélanie Ethier1, Bader Zarrouki1,2, Ghislaine Fontés1, Vincent Poitout1,2 1Montreal Diabetes Research Center, CRCHUM, 2Department of Medicine, University of Montreal A protocol for chronic infusions of glucose and Intralipid in rats is described. This model can be used to study the impact of nutrient excess on organ function and physiological parameters.
