2 articles published in JoVE
A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3 Alexa C. Robitaille*1,2,3, Mélissa K. Mariani*1,3, Audray Fortin1, Nathalie Grandvaux1,2,3 1CRCHUM - Research center, Centre Hospitalier de l'Université de Montréal, Université de Montréal, 2Department of Biochemistry and Molecular Medicine, Université de Montréal, 3Faculty of Medicine, Université de Montréal Here we describe a procedure allowing a detailed analysis of the phosphorylation-dependent activation of the IRF3 transcription factor. This is achieved through the combination of a high resolution SDS-PAGE and a native-PAGE coupled to immunoblots using multiple phosphospecific antibodies.
Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System. Mariana G. Bego1, Édouard A. Côté1, Éric A. Cohen1,2 1Laboratory of Human Retrovirology, Institut de Recherches Cliniques de Montréal (IRCM), 2Department of Microbiology, Infectiology, and Immunology, Université de Montréal Unlike cell-free HIV-1 particles, infected CD4+ T cells are effectively sensed by plasmacytoid dendritic cells (pDCs). This manuscript describes a method where peripheral blood mononuclear cells (PBMCs) or isolated pDCs are co-cultured with HIV-1 infected T cells to evaluate innate sensing by pDCs as assessed by release of type-I IFN.