Ontario Institute for Cancer Research 1 article published in JoVE Bioengineering Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-13C]pyruvate Soojin Kim1, Francisco Martinez-Santiesteban2, Timothy J. Scholl1,2,3 1Department of Medical Biophysics, Western University, 2Imaging Research Laboratory, Robarts Research Institute, Western University, 3Ontario Institute for Cancer Research We present a protocol to measure the magnetic field dependence of the spin-lattice relaxation time of 13C-enriched compounds, hyperpolarized by means of dynamic nuclear polarization, using fast field-cycled relaxometry. Specifically, we have demonstrated this with [1-13C]pyruvate, but the protocol could be extended to other hyperpolarized substrates.