3 articles published in JoVE
Tilt Testing with Combined Lower Body Negative Pressure: a "Gold Standard" for Measuring Orthostatic Tolerance Clare L. Protheroe1, Henrike (Rianne) J.C. Ravensbergen1, Jessica A. Inskip1, Victoria E. Claydon1 1Department of Biomedical Physiology and Kinesiology, Simon Fraser University We describe a "gold standard" for evaluating orthostatic tolerance (OT) using tilt testing with combined lower body negative pressure (LBNP). This can be combined with non-invasive evaluations of cardiovascular reflex control. Normal and abnormal responses are defined.
Avian Influenza Surveillance with FTA Cards: Field Methods, Biosafety, and Transportation Issues Solved Robert H.S. Kraus1, Pim van Hooft1, Jonas Waldenström2, Neus Latorre-Margalef2, Ronald C. Ydenberg1,3, Herbert H.T. Prins1 1Resource Ecology Group, Wageningen University, 2Section for Zoonotic Ecology and Epidemiology, School of Natural Sciences, Linnaeus University, 3Centre for Wildlife Ecology, Simon Fraser University A method to preserve, detect and sequence RNA from Avian Influenza Viruses was validated and extended using natural faecal samples from birds. This technique removes the necessity of maintaining a cool chain and handling of infectious viruses and can be applied in a 96-well high-throughput setup.
Live Imaging of Dense-core Vesicles in Primary Cultured Hippocampal Neurons David M. Kwinter1, Michael A. Silverman1 1Department of Biological Sciences, Simon Fraser University Live cell imaging is of particular utility when studying the dynamics of organelle trafficking. Here we describe a protocol for live imaging of dense-core vesicles in cultured neurons using wide-field fluorescence microscopy. This protocol is flexible and can be adapted to image other organelles such as mitochondria, endosomes, and peroxisomes.