3 articles published in JoVE
A Novel and Translational Rat Model of Concussion Combining Force and Rotation with In Vivo Cerebral Microdialysis Ian O. Massé1, Luc Moquin2, Chloé Provost1, Samuel Guay1, Alain Gratton2, Louis De Beaumont1 1Research Center, Hôpital du Sacré-Cœur de Montréal, 2Research Center, Douglas Institute Neurotransmitter alteration is a mechanism of neural dysfunction that occurs after concussion and contributes to the sometimes-catastrophic long-term consequences. This rat model combines microdialysis, allowing in vivo neurotransmitter quantification, with a weight-drop technique exerting rapid acceleration and deceleration of the head and torso, an important factor of human craniocerebral trauma.
How to Find Effects of Stimulus Processing on Event Related Brain Potentials of Close Others when Hyperscanning Partners Amanda Tardif1,3, Ashley Chau-Morris1,2, Zi Yue Wang1, Ehime Takahara1, Tim Hadjis1,2, Jean Debruille1, J. Bruno Debruille1,2,3 1Douglas Mental Health University Institute, 2Department of Psychiatry, McGill University, 3Department of Neurology and Neurosurgery, McGill University This protocol describes key steps involved in assessing the sensitivity of the brain of one person to the stimulus processing of a close other by selecting pairs of partners, recording their electroencephalogram (EEG) simultaneously and computing their event-related brain potentials (ERPs).
Preparation of Mouse Brain Tissue for Immunoelectron Microscopy Marie-Eve Tremblay1, Mustapha Riad2, Ania Majewska1 1Department of Neurobiology and Anatomy, University of Rochester, 2Douglas Mental Health University Institute We describe a protocol for transcardiac perfusion of mice, removal and sectioning of the brain, as well as immunoperoxidase staining, resin embedding, and ultrathin sectioning of the brain sections. Upon completion of these procedures, the immunostained material is ready for examination with transmission electron microscopy.