Instituto Tecnologico de Morelia 1 article published in JoVE Biology Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism Ivanna Karina Olivares-Marin1, Juan Carlos González-Hernández2, Carlos Regalado-Gonzalez1, Luis Alberto Madrigal-Perez3 1Department of Chemistry, Universidad Autónoma de Querétaro, 2Department of Biochemical Engineering, Instituto Tecnológico de Morelia, 3Department of Biochemical Engineering, Instituto Tecnológico Superior de Ciudad Hidalgo Here we present a protocol to estimate the respiratory and fermentative metabolism by fitting the exponential growth of Saccharomyces cerevisiae to the exponential growth equation. Calculation of the kinetic parameters allows for the screening of influences of substances/compounds on fermentation or mitochondrial respiration.
