2 articles published in JoVE
Three-dimensional Alginate-bead Culture of Human Pituitary Adenoma Cells Dulce Avila-Rodríguez1, Karina Paisano-Cerón1, Irene Valdovinos- Ramírez1, Carmen Solano-Agama1, Alma Ortiz-Plata2, María E. Mendoza-Garrido1 1Department of Physiology, Biophysics and Neurosciences, Center for Research and Advanced Studies (CINVESTAV-IPN), 2Department of Experimental Pathology, Nacional Institute of Neurology and Neurosurgery Three-dimensional culture in alginate beads, due to its simplicity and reproducibility, was chosen to maintain the pituitary adenoma cells. The procedures included an initial enzymatic digestion and mechanical dissociation of the tumor tissue, and the subsequent cell suspension was encapsulated in alginate beads.
Analysis of the Epithelial Damage Produced by Entamoeba histolytica Infection Abigail Betanzos1, Michael Schnoor2, Rosario Javier-Reyna1, Guillermina García-Rivera1, Cecilia Bañuelos3, Jonnatan Pais-Morales1, Esther Orozco1 1Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies of the National Polytechnic Institute, 2Department of Molecular Biomedicine, Center for Research and Advanced Studies of the National Polytechnic Institute, 3Agency for Knowledge Commercialization, Center for Research and Advanced Studies of the National Polytechnic Institute Human infection by Entamoeba histolytica leads to amoebiasis, a major cause of diarrhea in tropical countries. Infection is initiated by pathogen interactions with intestinal epithelial cells, provoking the opening of cell-cell contacts and consequently diarrhea, sometimes followed by liver infection. This article provides a model to assess the early host-pathogen interactions to improve our understanding of amoebiasis pathogenesis.