CIC-Instituto Politecnico Nacional (IPN) 1 article published in JoVE Bioengineering Automation of Bio-Atomic Force Microscope Measurements on Hundreds of C. albicans Cells Childérick Severac*1, Sergio Proa-Coronado*1,2,3, Cécile Formosa-Dague4, Adrian Martinez-Rivas3,5, Etienne Dague2 1ITAV-CNRS, Université de Toulouse, CNRS, Toulouse, France, 2LAAS-CNRS, Université de Toulouse, CNRS, Toulouse, France, 3ENCB, Instituto Politécnico Nacional (IPN), Mexico City, Mexico, 4TBI, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France, 5CIC, Instituto Politécnico Nacional (IPN), Mexico City, Mexico This protocol aims to automate AFM measurements on hundreds of microbial cells. First, microbes are immobilized into PDMS stamp microstructures and then force spectroscopy measurements are performed automatically on hundreds of immobilized cells.
