2 articles published in JoVE
Glass Wool Filters for Concentrating Waterborne Viruses and Agricultural Zoonotic Pathogens Hana T. Millen1, Jordan C. Gonnering1, Ryan K. Berg2, Susan K. Spencer3, William E. Jokela3, John M. Pearce4, Jackson S. Borchardt1, Mark A. Borchardt3 1Wisconsin Water Science Center, United States Geological Survey, 2University of Wisconsin – Madison, 3Agricultural Research Service, United States Department of Agriculture, 4Alaska Science Center, United States Geological Survey Glass wool filters have been used to concentrate waterborne viruses by a number of research groups around the world. Here we show a simple approach for constructing glass wool filters and demonstrate the filters are also effective in concentrating waterborne viral, bacterial and protozoan pathogens.
Rapid Diagnosis of Avian Influenza Virus in Wild Birds: Use of a Portable rRT-PCR and Freeze-dried Reagents in the Field John Y. Takekawa1, Nichola J. Hill1,2, Annie K. Schultz1, Samuel A. Iverson1, Carol J. Cardona3,4, Walter M. Boyce2, Joseph P. Dudley5 1USGS Western Ecological Research Center, 2Wildlife Health Center, University of California, Davis, 3Department of Population Health and Reproduction, University of California, Davis, 4Department of Veterinary and Biomedical Sciences, University of Minnesota, 5Science Applications International Corporation This study describes diagnosis of avian influenza in wild birds using a portable rRT-PCR system. The method takes advantage of freeze-dried reagents to screen wild birds in a non-laboratory setting, typical of an outbreak scenario. Use of molecular tools provides accurate and sensitive alternatives for rapid diagnosis.