2 articles published in JoVE
Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty Hassan N. Tausif1, Lauren Johnson2, Michael Titus2, Kyle Mavin2, Navasuja Chandrasekaran1, Maria A. Woodward1, Roni M. Shtein1, Shahzad I. Mian1 1Department of Ophthalmology, University of Michigan, 2MidWest Eye Banks Endothelial keratoplasty is a surgical technique continuously evolving as advancements result in transplantation of thinner grafts with each succession1. Here we present a technique for controlled manual tissue dissection to allow safe and repeatable separation of endothelium and Descemet's membranes from donor corneoscleral buttons for transplantation2.
Corneal Donor Tissue Preparation for Endothelial Keratoplasty Maria A. Woodward1, Michael Titus2, Kyle Mavin2, Roni M. Shtein1 1Department of Ophthalmology, University of Michigan, 2MidWest Eye Banks Endothelial corneal transplantation is a surgical technique for treatment of posterior corneal diseases. Mechanical microkeratome dissection to prepare tissue results in thinner, more symmetric grafts with less endothelial cell loss and improved outcomes. Dissections can be performed at the eye bank prior to corneal transplantation surgery.