University of Wisconsin, School of Veterinary Medicine 1 article published in JoVE Developmental Biology Mass Isolation and In Vitro Cultivation of Intramolluscan Stages of the Human Blood Fluke Schistosoma Mansoni Nathalie Dinguirard1, Codie Heinemann1, Timothy P. Yoshino1 1Department of Pathobiological Sciences, University of Wisconsin, School of Veterinary Medicine This article describes a protocol for the large-scale axenic isolation and collection of free-swimming miracidia of the human blood fluke Schistosoma mansoni and their subsequent processing for introduction into in vitro culture.