2 articles published in JoVE
Preparation of Primary Mixed Glial Cultures from Adult Mouse Spinal Cord Tissue Jennifer T. Malon1, Ling Cao1 1Department of Biomedical Sciences, College of Osteopathic Medicine, University of New England The development of neuropathic pain involves pathological changes of spinal cord glial cells. A reliable glial culture system derived from adult spinal cord tissue and designed for studying these cells in vitro is lacking. Therefore, we show here how to establish primary mixed glial cultures from adult mouse spinal cord tissue.
Measurement of Larval Activity in the Drosophila Activity Monitor Aidan L. McParland1, Taylor L. Follansbee1, Geoffrey K. Ganter1 1Department of Biology, University of New England This report describes a method for measuring Drosophila larval activity using the TriKinetics Drosophila Activity Monitor. The device employs infrared beams to detect movements of up to 16 individual animals. Data can be analyzed to represent motion parameters including rates and the positions of the animals within the assay chambers.