Renovo Neural Inc. 2 articles published in JoVE Neuroscience Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy Konark Mukherjee1, Helen R. Clark1, Vrushali Chavan1, Emily K. Benson2, Grahame J. Kidd2, Sarika Srivastava1 1Virginia Tech Carilion Research Institute, 2Renovo Neural Incorporated Mitochondrial visualization and analysis from mammalian brain tissue is a challenging task. Here, we describe how three dimensional (3D) reconstruction analysis from the serial block-face scanning electron microscopy (SBFSEM) can be used to gain insights on the morphological and volumetric analysis of this critical energy generating organelle. Biology Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell Diego Bohórquez1, Fariha Haque2, Satish Medicetty3, Rodger A. Liddle1 1Department of Medicine, Duke University Medical Center, 2Department of Chemistry, University of North Carolina - Chapel Hill, 3Renovo Neural Incorporated Here, we introduce a method, cocem3D, to unveil the ultrastructure of a specific cell in its native tissue by bridging confocal and serial block-face scanning electron microscopy.
