National Center for Advancing Translational Sciences 1 article published in JoVE Biology High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells Bruce Nguyen Tran1, Eunkeu Oh2, Kimihiro Susumu2,3, Mason Wolak2, Kirill Gorshkov1 1National Center for Advancing Translational Sciences, 2Optical Sciences Division, Code 5600, Naval Research Laboratory, 3Jacobs Corporation In this protocol, quantum dots conjugated to recombinant SARS-CoV-2 spike enable cell-based assays to monitor spike binding to hACE2 at the plasma membrane and subsequent endocytosis of the bound proteins into the cytoplasm.
