2 articles published in JoVE
Caenorhabditis Sieve: A Low-tech Instrument and Methodology for Sorting Small Multicellular Organisms Skyler Hunter*1,2, Malabika Maulik*3, Courtney Scerbak4, Elena Vayndorf2, Barbara E. Taylor5 1Department of Biology and Wildlife, University of Alaska Fairbanks, 2Institute of Arctic Biology, University of Alaska Fairbanks, 3Department of Chemistry and Biochemistry, University of Alaska Fairbanks, 4Departments of Biology and Chemistry, Earlham College, 5Department of Biological Sciences, College of Natural Science and Mathematics, California State University Long Beach The current protocol includes a methodology for the sorting and cleaning of age-matched populations of Caenorhabditis elegans. It uses a simple, inexpensive, and efficient custom-made tool to obtain a large experimental population of nematodes for research.
Growth and Differentiation of Adult Hippocampal Arctic Ground Squirrel Neural Stem Cells Kelly L. Drew1, Rebecca C. McGee2, Matthew S. Wells3, Judith A. Kelleher-Andersson4 1Alaska Basic Neuroscience Program, Institute of Arctic Biology, University of Alaska at Fairbanks, 2Department Biochemistry, Hood College, 3Department of Cell Biology, Neuronascent, Inc., 4Research and Development, Neuronascent, Inc. Neural stem cells were prepared from the hippocampus of adult non-hibernating yearling Arctic ground squirrels (AGS). These neural stem cells can be expanded through numerous passages, differentiated and maintained as a nearly 50:50 neuron to glial culture.