3 articles published in JoVE
Using Enzyme-based Biosensors to Measure Tonic and Phasic Glutamate in Alzheimer's Mouse Models Holly C. Hunsberger1, Sharay E. Setti2, Ryan T. Heslin2, Jorge E. Quintero3, Greg A. Gerhardt3, Miranda N. Reed2 1Department of Psychology, West Virginia University, 2Department of Drug Discovery & Development, Auburn University, 3Department of Neuroscience, University of Kentucky Medical Center Here, we describe the setup, software navigation, and data analysis for a spatially and temporally precise method of measuring tonic and phasic extracellular glutamate changes in vivo using enzyme-linked microelectrode arrays (MEA).
Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria Rajesh Guntupalli1, Iryna Sorokulova1, Eric Olsen2, Ludmila Globa1, Oleg Pustovyy1, Vitaly Vodyanoy1 1Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, 2Clinical Research Laboratory, 81st Medical Group, Keesler Air Force Base Lytic phage biosensors and antibody beads are able to discriminate between methicillin resistant (MRSA) and sensitive staphylococcus bacteria. The phages were immobilized by a Langmuir-Blodgett method onto a surface of a quartz crystal microbalance sensor and worked as broad range staphylococcus probes. Antibody beads recognize MRSA.
Isolation and Culture of Hippocampal Neurons from Prenatal Mice Michael L. Seibenhener1, Marie W. Wooten1 1Department of Biological Sciences, Auburn University We provide a protocol for the culture of highly purified hippocampal neurons from prenatal mouse brains without the use of a feeder glial cell layer.