2 articles published in JoVE
A Simplified Method for Ultra-Low Density, Long-Term Primary Hippocampal Neuron Culture Zhongming Lu*1,2, Mariel Piechowicz*1, Shenfeng Qiu1 1Department of Basic Medical Sciences, University of Arizona College of Medicine-Phoenix, 2Jiangsu Provincial Center for Disease Control and Prevention Low density cultures of primary hippocampal neurons usually require glia feeder layer to supply neurotrophic factors and sustain longevity. We describe here a simplified method to culture ultra-low density neurons on glass coverslips in the presence of a high density neuronal feeder layer, which facilitates investigation of specific neuronal-autonomous mechanisms.
Culturing and Applications of Rotating Wall Vessel Bioreactor Derived 3D Epithelial Cell Models Andrea L. Radtke1, Melissa M. Herbst-Kralovetz1 1Basic Medical Sciences, University of Arizona College of Medicine - Phoenix A rotating cell culture system that allows epithelial cells to grow under physiological conditions resulting in 3-D cellular aggregate formation is described. The aggregates generated display in vivo-like characteristics not observed in conventional culture models and serve as a more accurate organotypic model system for a multitude of scientific investigations.