3 articles published in JoVE
Intratracheal Inoculation of Fischer 344 Rats with Francisella tularensis Jesse Q. Nguyen1, Xhavit Zogaj1, Aanuoluwa A. Adelani1, Ping Chu2, Jieh-Juen Yu1, Bernard P. Arulanandam1, Karl E. Klose1 1South Texas Center for Emerging Infectious Diseases, University of Texas San Antonio, 2Midwestern University This protocol describes intratracheal inoculations of Fischer 344 rats with Francisella tularensis. This procedure mimics pulmonary exposure of humans to this potential biothreat agent and can be used to test vaccine and therapeutic efficacy against pulmonary tularemia.
Creating and Applying a Reference to Facilitate the Discussion and Classification of Proteins in a Diverse Group D. Ellen K. Tarr1 1Department of Microbiology and Immunology, Arizona College of Osteopathic Medicine, Midwestern University The goal of this protocol is to develop a reference for divergent proteins in a group that lacks coherent criteria for nomenclature and classification. This reference will facilitate analyses and discussion of the group as a whole and can be used in addition to established names.
Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts Yuriana Aguilar-Sanchez*1, Diego Fainstein*2,3, Rafael Mejia-Alvarez4, Ariel L. Escobar5 1School of Natural Sciences, University of California, Merced, 2Centro de Investigaciones Cardiovasculares, Universidad de la Plata and Conicet, 3Facultad de Ingenieria, Universidad Nacional de Entre Rios, 4Department of Physiology, Midwestern University, 5School of Engineering, University of California, Merced In the heart, molecular events coordinate the electrical and contractile function of the organ. A set of local field fluorescence microscopy techniques presented here enables the recording of cellular variables in intact hearts. Identifying mechanisms defining the cardiac function is critical in understanding how the heart works under pathological situations.