4 articles published in JoVE
A Heterotopic Mouse Model for Studying Laryngeal Transplantation Maeve M. Kennedy*1, Egehan Salepci*1,2, Cheryl Myers1, Marshall Strome3,4, David G. Lott1,5 1Head and Neck Regenerative Medicine Laboratory, Center for Regenerative Medicine, Mayo Clinic Arizona, 2Department of Otorhinolaryngology, University of Health Sciences, Sisli Hamidiye Etfal Training and Research Hospital, 3Department of Otolaryngology, Vanderbilt University, 4Cleveland Clinic Head and Neck Institute, 5Division of Laryngology, Department of Otolaryngology Head and Neck Surgery, Mayo Clinic Arizona The aim of this manuscript is to describe the microsurgical steps required to perform a heterotopic laryngeal transplant in mice. The advantages of this mouse model compared to other animal models of laryngeal transplantation are its cost-effectiveness and the availability of immunologic assays and data.
Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer Lee Wisner1, Brandon Larsen2, Alanna Maguire1 1Department of Research, Mayo Clinic, 2Department of Laboratory Medicine and Pathology, Mayo Clinic This protocol outlines the tape method on how to manually construct a tissue microarray using FFPE donor blocks of differing depths.
Enhancing Tumor Content through Tumor Macrodissection Lee Wisner1, Brandon Larsen2, Alanna Maguire1 1Department of Research, Mayo Clinic, 2Department of Laboratory Medicine and Pathology, Mayo Clinic This protocol presents a method to increase the percent tumor content of formalin-fixed paraffin-embedded tissue samples.
Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion James J. Faust1,2, Wayne Christenson3,4,5, Kyle Doudrick6, John Heddleston7, Teng-Leong Chew7, Marko Lampe8, Arnat Balabiyev1,2, Robert Ros3,4,5, Tatiana P. Ugarova1,2 1Center for Metabolic and Vascular Biology, Mayo Clinic, 2Molecular and Cellular Biosciences, School of Life Sciences, Arizona State University, 3Department of Physics, Arizona State University, 4Center for Biological Physics, Arizona State University, 5Biodesign Institute, Arizona State University, 6Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, 7Advanced Imaging Center, HHMI Janelia Research Campus, 8Advanced Light Microscopy Facility, European Molecular Biology Laboratory This protocol describes the fabrication of optical-quality glass surfaces adsorbed with compounds containing long-chain hydrocarbons that can be used to monitor macrophage fusion of living specimens and enables super-resolution microscopy of fixed specimens.