St. Joseph's Hospital and Medical Center 2 articles published in JoVE Immunology and Infection Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages Deepak K. Nayak1, Oscar Mendez1, Sara Bowen2, Thalachallour Mohanakumar1 1 This communication describes methodologies for isolation and culture of alveolar macrophages from humans and murine models for experimental purposes. Biology An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells Landon J. Inge1, Aaron J. Fowler1, Ross M. Bremner1 1 There is a discernible need for improved information on molecular drivers of Barrett’s Esophagus. Immunofluorescent staining is a useful technique for understanding the effects of cell signaling on cell morphology. We present a simple, effective protocol for the use of immunofluorescent staining to assess therapeutic treatment in Barrett’s Esophagus cells.
