3 articles published in JoVE
Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing Lisa Couper1, Andrea Swei1 1Department of Biology, San Francisco State University Here we present a next-generation sequencing protocol for 16S rRNA sequencing which enables identification and characterization of microbial communities within vectors. This method involves DNA extraction, amplification and barcoding of samples through PCR, sequencing on a flow-cell, and bioinformatics to match sequence data to phylogenetic information.
Using Microwave and Macroscopic Samples of Dielectric Solids to Study the Photonic Properties of Disordered Photonic Bandgap Materials Seyed Reza Hashemizad1, Sam Tsitrin1, Polin Yadak1, Yingquan He1, Daniel Cuneo1, Eric Paul Williamson1, Devin Liner1, Weining Man1 1Department of Physics and Astronomy, San Francisco State University Disordered structures offer new mechanisms for forming photonic bandgaps and unprecedented freedom in functional-defect designs. To circumvent the computational challenges of disordered systems, we construct modular macroscopic samples of the new class of PBG materials and use microwaves to characterize their scale-invariant photonic properties, in an easy and inexpensive manner.
Myo-mechanical Analysis of Isolated Skeletal Muscle Peter E. Oishi1,2, Sompob Cholsiripunlert3, Wenhui Gong2, Anthony J. Baker4, Harold S. Bernstein1,2,5 1Cardiovascular Research Institute, University of California San Francisco, 2Department of Pediatrics, University of California San Francisco, 3Department of Biology, San Francisco State University, 4Department of Medicine, University of California San Francisco, 5Eli and Edythe Broad Center of Regeneration Medicine & Stem Cell Research, University of California San Francisco To assess the in vivo effects of therapeutic interventions for muscle disease, methods are needed to quantitate force generation and fatigability in treated muscle. We detail an approach to evaluating myo-mechanical properties in explanted mouse hindlimb muscle. This analysis provides a robust approach to quantitating the effects of genetic modification on muscle function, as well as comparison of therapies in mouse models of muscle disease.