3 articles published in JoVE
Evaluation of Stem Cell Therapies in a Bilateral Patellar Tendon Injury Model in Rats John R. Wagner1, Takashi Taguchi1, Jane Y. Cho2, Chandrashekhar Charavaryamath3, Dominique J. Griffon1 1College of Veterinary Medicine, Western University of Health Sciences, 2Applied Medical, 3College of Veterinary Medicine, Iowa State University This paper describes the preparation and evaluation of umbilical cord matrix-derived mesenchymal stem cells spheroids with a bilateral patellar tendon defect model in a rat. This model was associated with an acceptable morbidity and was found to detect differences between untreated and treated tendons, and between the two treatments tested.
The Optokinetic Response as a Quantitative Measure of Visual Acuity in Zebrafish Donald Joshua Cameron1,2, Faydim Rassamdana1, Peony Tam1, Kathleen Dang1, Carolina Yanez3, Saman Ghaemmaghami3, Mahsa Iranpour Dehkordi3 1College of Optometry, Western University of Health Sciences, 2Molecular Neurobiology Group, Western University of Health Sciences, 3Graduate College of Biomedical Sciences, Western University of Health Sciences Zebrafish have been a valuable tool for many research areas. Here we demonstrate a method to elicit a visual response and calculate a functional visual acuity in the adult zebrafish.
Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5 Maria Pia Testa1, Omar Alvarado1, Andrea Wournell1, Jonathan Lee2, Frederick T. Guilford3, Steven H. Henriksen2, Tom R. Phillips1,2 1College of Veterinary Medicine, Western University of Health Sciences, 2Graduate College of Biomedical Sciences, Western University of Health Sciences, 3ReadiSorb, Products A screening method to detect oxidative cellular environments is to measure the oxidation of CM-H2DCFDA. Once oxidized within a cell, CM-H2DCFDA changes from non-fluorescent into a fluorescent compound. This change in fluorescence is measured by flow cytometry and indicates the number of cells in an oxidative environment.