3 articles published in JoVE
Modeling Paracrine Noncanonical Wnt Signaling In Vitro Omar Toubat1, Jongkyu Choi1, S. Ram Kumar1,2,3 1Department of Surgery, Keck School of Medicine of USC, 2Department of Pediatrics, Keck School of Medicine of USC, 3 The present study outlines a highly reproducible and tractable method to study paracrine noncanonical Wnt signaling events in vitro. This protocol was applied to evaluate the impact of paracrine Wnt5a signaling in murine neural crest cells and myoblasts.
Fast and Efficient Expression of Multiple Proteins in Avian Embryos Using mRNA Electroporation Martin Tran1, Mohit Dave2,3, Rusty Lansford1,2,3 1Department of Biological Sciences, University of Southern California, Los Angeles, 2 We report messenger RNA (mRNA) electroporation as a method that permits fast and efficient expression of multiple proteins in the quail embryo model system. This method can be used to fluorescently label cells and record their in vivo movements by time-lapse microscopy shortly after electroporation.
Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching Gianni Carraro1, Pierre-Marie del Moral1, David Warburton1 1Saban Research Institute, Childrens Hospital Los Angeles Early embryonic lung organ culture is a very useful system to study epithelial-mesenchymal interactions. Both epithelial and mesenchymal morphogenesis proceeds under specific conditions that can be readily manipulated in this system.