La Jolla Institute for Allergy and Immunology View Institution's Website 5 articles published in JoVE Immunology and Infection Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells Yanfang Peipei Zhu1, Lindsey Padgett1, Huy Q. Dinh1, Paola Marcovecchio1, Runpei Wu1, Denise Hinz2, Cheryl Kim2, Catherine C. Hedrick1 1Division of Inflammation Biology, La Jolla Institute for Immunology, 2Flow Cytometry Core Facility, La Jolla Institute for Immunology Here, we present a protocol to process fresh bone marrow (BM) isolated from mouse or human for high-dimensional mass cytometry (Cytometry by Time-Of-Flight, CyTOF) analysis of neutrophil-lineage cells. Immunology and Infection Mouse Body Temperature Measurement Using Infrared Thermometer During Passive Systemic Anaphylaxis and Food Allergy Evaluation Yu Kawakami1, Rachel Sielski1, Toshiaki Kawakami1 1Division of Cell Biology, La Jolla Institute for Allergy and Immunology Here we present a new method to accurately measure body temperature differences in passive systemic anaphylaxis (PSA) and food allergy mouse models using an infrared thermometer. This procedure has been accurately duplicated in previous PSA results. Immunology and Infection Flow Cytometry Analysis of Immune Cells Within Murine Aortas Matthew J. Butcher1, Margo Herre1, Klaus Ley2, Elena Galkina1 1Deptartment of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, 2Division of Inflammation Biology, LaJolla Institute for Allergy and Immunology This paper presents a flow cytometry-based method to investigate the immune composition of aortas. The paper also illustrates an additional technique that allows examining surrounding adventitia and vessel wall separately. This method opens possibilities to perform phenotypical analyses of aortic leukocytes and apply several immunological assays for atherosclerosis studies. Biology Interview: Glycolipid Antigen Presentation by CD1d and the Therapeutic Potential of NKT cell Activation Mitchell Kronenberg1 1Department of Developmental Immunology, La Jolla Institute for Allergy and Immunology Natural Killer T cells (NKT) are critical determinants of the immune response to cancer, regulation of autioimmunity, clearance of infection, and the development of artheriosclerotic plaques. In this interview, Mitch Kronenberg discusses his laboratory's efforts to understand the mechanism through which NKT cells are activated by glycolipid antigens. Biology A Method For Production of Recombinant mCD1d Protein in Insect Cells. Archana Khurana1, Mitchell Kronenberg1 1Department of Developmental Immunology, La Jolla Institute for Allergy and Immunology A Method to prepare Insect cells and infect them with baculovirus for the the purpose of production of recombinant mCD1d proteinand generating mCD1d tetramers.