3 articles published in JoVE
Flow Cytometry Analysis of Immune Cells Within Murine Aortas Matthew J. Butcher1, Margo Herre1, Klaus Ley2, Elena Galkina1 1Deptartment of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, 2Division of Inflammation Biology, LaJolla Institute for Allergy and Immunology This paper presents a flow cytometry-based method to investigate the immune composition of aortas. The paper also illustrates an additional technique that allows examining surrounding adventitia and vessel wall separately. This method opens possibilities to perform phenotypical analyses of aortic leukocytes and apply several immunological assays for atherosclerosis studies.
Interview: Glycolipid Antigen Presentation by CD1d and the Therapeutic Potential of NKT cell Activation Mitchell Kronenberg1 1Department of Developmental Immunology, La Jolla Institute for Allergy and Immunology Natural Killer T cells (NKT) are critical determinants of the immune response to cancer, regulation of autioimmunity, clearance of infection, and the development of artheriosclerotic plaques. In this interview, Mitch Kronenberg discusses his laboratory's efforts to understand the mechanism through which NKT cells are activated by glycolipid antigens.
A Method For Production of Recombinant mCD1d Protein in Insect Cells. Archana Khurana1, Mitchell Kronenberg1 1Department of Developmental Immunology, La Jolla Institute for Allergy and Immunology A Method to prepare Insect cells and infect them with baculovirus for the the purpose of production of recombinant mCD1d proteinand generating mCD1d tetramers.