3 articles published in JoVE
Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts Yuriana Aguilar-Sanchez*1, Diego Fainstein*2,3, Rafael Mejia-Alvarez4, Ariel L. Escobar5 1School of Natural Sciences, University of California, Merced, 2Centro de Investigaciones Cardiovasculares, Universidad de la Plata and Conicet, 3Facultad de Ingenieria, Universidad Nacional de Entre Rios, 4Department of Physiology, Midwestern University, 5School of Engineering, University of California, Merced In the heart, molecular events coordinate the electrical and contractile function of the organ. A set of local field fluorescence microscopy techniques presented here enables the recording of cellular variables in intact hearts. Identifying mechanisms defining the cardiac function is critical in understanding how the heart works under pathological situations.
Rapid Fibroblast Removal from High Density Human Embryonic Stem Cell Cultures William S. Turner1, Kara E. McCloskey1 1Department of Engineering, University of California, Merced Despite ongoing efforts to transition cultures to feeder-free conditions, the derivation and culture of human embryonic stem cells (hESC) remain largely dependent on co-cultures with mouse embryonic feeders (MEFs). Here, we show a novel methodology for rapidly removing feeders from hESC cultures prior to experimentation.
Shrinky-Dink Hanging Drops: A Simple Way to Form and Culture Embryoid Bodies Chi-Shuo Chen1, Jonathan Pegan1, Jesus Luna1, Bing Xia1, Kara McCloskey1, Wei-chun Chin1, Michelle Khine1 1School of Engineering, University of California Merced - UC Merced We show a simple and rapid method to load pre-defined numbers of cells into microfabricated wells and maintain them for embryoid body development.