2 articles published in JoVE
High-throughput Screening for Protein-based Inheritance in S. cerevisiae James S. Byers1, Daniel F. Jarosz1,2 1Department of Developmental Biology, Stanford University School of Medicine, 2Department of Chemical and Systems Biology, Stanford University School of Medicine This protocol describes a high-throughput methodology to functionally screen for protein-based inheritance in S. cerevisiae.
A Human Glioblastoma Organotypic Slice Culture Model for Study of Tumor Cell Migration and Patient-specific Effects of Anti-Invasive Drugs Jonathon J. Parker1, Marcela Lizarraga2, Allen Waziri2, Kara M. Foshay2 1Department of Neurosurgery, Stanford University Hospital, Stanford University School of Medicine, 2Inova Neuroscience Institute Current ex vivo models of glioblastoma (GBM) are not optimized for physiologically relevant study of human tumor invasion. Here, we present a protocol for generation and maintenance of organotypic slice cultures from fresh human GBM tissue. A description of time-lapse microscopy and quantitative cell migration analysis techniques is provided.