2 articles published in JoVE
A Simple and Low-cost Assay for Measuring Ambulation in Mouse Models of Muscular Dystrophy Elizabeth M. Gibbs1,2, Rachelle H. Crosbie-Watson1,2,3,4 1Department of Integrative Biology and Physiology, University of California, 2Center for Duchenne Muscular Dystrophy, University of California, 3Department of Neurology, David Geffen School of Medicine, University of California, 4Molecular Biology Institute, University of California This protocol describes a flexible, low-cost system for measuring mouse ambulation in an open field activity assay. We show that a 6-minute ambulation assay based on this system detects a decrease in voluntary movement in mdx mice, and accurately distinguishes improvement in a muscle-specific rescue of these animals.
High-throughput Parallel Sequencing to Measure Fitness of Leptospira interrogans Transposon Insertion Mutants During Golden Syrian Hamster Infection Kristel Lourdault1,2, James Matsunaga1,2, Karen V. Evangelista1,2, David A. Haake1,2,3,4 1Veterans Affairs Greater Los Angeles Healthcare System, 2Departments of Medicine, David Geffen School of Medicine at University of California Los Angeles, 3Departments of Urology, David Geffen School of Medicine at University of California Los Angeles, 4Departments of Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles We describe here a technique that combines transposon mutagenesis with high-throughput sequencing to identify and quantify transposon leptospiral mutants in tissues after a challenge of hamsters. This protocol can be used to screen mutants for survival and dissemination in animals and can also be applied to in vitro studies.