UCLA 4 articles published in JoVE Biochemistry A Knowledge Graph Approach to Elucidate the Role of Organellar Pathways in Disease via Biomedical Reports Alexander R. Pelletier1,2,3, Dylan Steinecke1,3,4, Dibakar Sigdel1, Irsyad Adam1, J. Harry Caufield1, Vladimir Guevara-Gonzalez1, Joseph Ramirez1, Aarushi Verma1, Kaitlyn Bali1, Katherine Downs1, Wei Wang1,2,3, Alex Bui3,4, Peipei Ping1,2,3,4,5 1Department of Physiology, UCLA School of Medicine, 2Scalable Analytics Institute (ScAi) at Department of Computer Science, UCLA School of Engineering, 3NIH BRIDGE2AI Center at UCLA & NHLBI Integrated Cardiovascular Data Science Training Program, UCLA, 4Medical Informatics, University of California at Los Angeles (UCLA), 5Department of Medicine (Cardiology), UCLA School of Medicine A computational protocol, CaseOLAP LIFT, and a use case are presented for investigating mitochondrial proteins and their associations with cardiovascular disease as described in biomedical reports. This protocol can be easily adapted to study user-selected cellular components and diseases. Bioengineering Time-Resolved In Vivo Measurement of Neuropeptide Dynamics by Capacitive Immunoprobe in Porcine Heart Nicholas Kluge1, Shyue-An Chan1, Jeffrey L. Ardell2,3, Corey Smith1 1Department of Physiology and Biophysics, Case Western Reserve University, 2University of California Los Angeles (UCLA) Cardiac Arrhythmia Center, David Geffen School of Medicine, 3UCLA Neurocardiology Research Program of Excellence, UCLA Established immunochemical methods to measure peptide transmitters in vivo rely on microdialysis or bulk fluid draw to obtain the sample for offline analysis. However, these suffer from spatiotemporal limitations. The present protocol describes the fabrication and application of a capacitive immunoprobe biosensor that overcomes the limitations of the existing techniques. Chemistry Optimization of Radiochemical Reactions using Droplet Arrays Alejandra Rios1,2, Travis S. Holloway2,3, Jia Wang2,4, R. Michael van Dam1,2,3,4 1Physics and Biology in Medicine Interdepartmental Graduate Program, University of California Los Angeles (UCLA), 2Crump Institute of Molecular Imaging, UCLA, 3Department of Molecular & Medical Pharmacology, David Geffen School of Medicine, 4Department of Bioengineering, UCLA This method describes the use of a novel high-throughput methodology, based on droplet chemical reactions, for the rapid and economical optimization of radiopharmaceuticals using nanomole amounts of reagents. Genetics Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts Mithun Mitra1,2, Ha Neul Lee1,2,3, Hilary A. Coller1,2,3 1Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, 2Department of Biological Chemistry, David Geffen School of Medicine, 3Molecular Biology Institute Interdepartmental Program, University of California, Los Angeles We describe a protocol for generating proliferating and quiescent primary human dermal fibroblasts, monitoring transcript decay rates, and identifying differentially decaying genes.