3 articles published in JoVE
Drawing and Hydrophobicity-patterning Long Polydimethylsiloxane Silicone Filaments Katherine Snell1, Isabelle Lopez2, Brandon Louie1, Roxanna Kiessling1, Babak Sanii1,2,3 1Keck Science Department, Claremont McKenna College, 2Keck Science Department, Scripps College, 3Keck Science Department, Pitzer College Here, we present a protocol to produce long filaments of polydimethylsiloxane (PDMS) silicone by gravity-drawing through a furnace. Filaments are on the order of hundreds of micrometers in diameter and tens of centimeters in length and are hydrophobically patternable via an Arduino-controlled corona discharge system.
Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY Dustin G. Roberts1, Hadley B. Johnsonbaugh1, Rory D. Spence2, Allan MacKenzie-Graham1 1Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles, 2W.M. Keck Science Department, Claremont McKenna, Pitzer & Scripps Colleges Optical clearing techniques are revolutionizing the way tissues are visualized. In this report we describe modifications of the original Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging-compatible Tissue-hYdrogel (CLARITY) protocol that yields more consistent and less expensive results.
Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes Amanda M. Larracuente1, Patrick M. Ferree2 1Department of Biology, University of Rochester, 2W. M. Keck Science Department, Claremont McKenna, Pitzer, and Scripps Colleges Here, we present a simple method for performing fluorescence DNA in situ hybridization (DNA ISH) to visualize repetitive heterochromatic sequences on slide-mounted chromosomes. The method requires minimal reagents and it is versatile for use with short or long probes, different tissues, and detection with fluorescence or non-fluorescence-based signals.