2 articles published in JoVE
Immunophenotyping of Orthotopic Homograft (Syngeneic) of Murine Primary KPC Pancreatic Ductal Adenocarcinoma by Flow Cytometry Xiaoyu An*1,2, Xuesong Ouyang*1, Hui Zhang1, Tingting Li1, Yu-yang Huang1, Zhiyuan Li1, Demin Zhou2, Qi-Xang Li1,2 1Crown Bioscience Inc., 2State Key Laboratory of Natural and Biomimetic Drugs, Peking University The experimental procedure on the immunophenotyping of murine orthotopic PDAC homografts aims at profiling the tumor immuno-microenvironment. Tumors are orthotopically implanted via surgery. Tumors of 200–600 mm3 in size were harvested and dissociated to prepare single-cell suspensions, followed by multi-immune marker FACS analysis using different fluorescently-labeled antibodies.
Characterization of Metabolic Status in Nonhuman Primates with the Intravenous Glucose Tolerance Test Michael Staup1, George Aoyagi1, TeQuana Bayless1, Yixin Wang1, Keefe Chng1 1Cardiovascular and Metabolism Group, Crown Bioscience The goal of this protocol is to present a standard method to perform intravenous glucose tolerance tests (IVGTTs) to assess glycemic control in nonhuman primates and assess their metabolic status from healthy to dysmetabolic.