3 articles published in JoVE
Creating Matched In vivo/In vitro Patient-Derived Model Pairs of PDX and PDX-Derived Organoids for Cancer Pharmacology Research Xiaoxi Xu1, Limei Shang1, Philip Wang2, Jun Zhou2, Xuesong Ouyang2, Meiling Zheng1, Binchen Mao2, Likun Zhang2, Bonnie Chen1, Jingjing Wang2, Jing Chen3, Wubin Qian2, Sheng Guo2, Yujun Huang2, Qi-Xiang Li3 1Crown Bioscience Inc., Changping District, Beijing, China, 2Crown Bioscience Inc., Taicang, Jiangsu, China, 3Crown Bioscience Inc., San Diego, CA, USA A method is described to create organoids using patient-derived xenografts (PDX) for in vitro screening, resulting in matched pairs of in vivo/in vitro models. PDX tumors were harvested/processed into small pieces mechanically or enzymatically, followed by the Clevers’ method to grow tumor organoids that were passaged, cryopreserved and characterized against the original PDX.
Immunophenotyping of Orthotopic Homograft (Syngeneic) of Murine Primary KPC Pancreatic Ductal Adenocarcinoma by Flow Cytometry Xiaoyu An*1,2, Xuesong Ouyang*1, Hui Zhang1, Tingting Li1, Yu-yang Huang1, Zhiyuan Li1, Demin Zhou2, Qi-Xang Li1,2 1Crown Bioscience Inc., 2State Key Laboratory of Natural and Biomimetic Drugs, Peking University The experimental procedure on the immunophenotyping of murine orthotopic PDAC homografts aims at profiling the tumor immuno-microenvironment. Tumors are orthotopically implanted via surgery. Tumors of 200–600 mm3 in size were harvested and dissociated to prepare single-cell suspensions, followed by multi-immune marker FACS analysis using different fluorescently-labeled antibodies.
Characterization of Metabolic Status in Nonhuman Primates with the Intravenous Glucose Tolerance Test Michael Staup1, George Aoyagi1, TeQuana Bayless1, Yixin Wang1, Keefe Chng1 1Cardiovascular and Metabolism Group, Crown Bioscience The goal of this protocol is to present a standard method to perform intravenous glucose tolerance tests (IVGTTs) to assess glycemic control in nonhuman primates and assess their metabolic status from healthy to dysmetabolic.