2 articles published in JoVE
A Semi-high-throughput Imaging Method and Data Visualization Toolkit to Analyze C. elegans Embryonic Development Renat N. Khaliullin1,2,3, Jeffrey M. Hendel1,2, Adina Gerson-Gurwitz1,2, Shaohe Wang1,4,5, Stacy D. Ochoa1,6, Zhiling Zhao1,7, Arshad Desai1,2, Karen Oegema1,2, Rebecca A. Green1,2 1Ludwig Institute for Cancer Research, San Diego, 2Department of Cellular and Molecular Medicine, University of California, San Diego, 3Recursion Pharmaceuticals, 4Biomedical Sciences Graduate Program, University of California, San Diego, 5Cell Biology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, 6Department of Biology, San Diego State University, 7Developmental and Stem Cell Biology Graduate Program, University of California, San Francisco This work describes a semi-high-throughput protocol that allows simultaneous 3D time-lapse imaging of embryogenesis in 80–100 C. elegans embryos in a single overnight run. Additionally, image processing and visualization tools are included to streamline data analysis. The combination of these methods with custom reporter strains enables detailed monitoring of embryogenesis.
Fluorescence Molecular Tomography for In Vivo Imaging of Glioblastoma Xenografts Jorge A. Benitez*1, Ciro Zanca*1, Jianhui Ma1, Webster K. Cavenee1,2,3, Frank B. Furnari1,2,4 1Ludwig Institute for Cancer Research, 2Moores Cancer Center, School of Medicine, University of California, San Diego, 3Department of Pathology, School of Medicine, University of California, San Diego, 4Department of Medicine, School of Medicine, University of California, San Diego Orthotopic intracranial injection of tumor cells has been used in cancer research to study brain tumor biology, progression, evolution, and therapeutic response. Here we present fluorescence molecular tomography of tumor xenografts, which provides real-time intravital imaging and quantification of a tumor mass in preclinical glioblastoma models.