2 articles published in JoVE
Three-dimensional Organotypic Cultures of Vestibular and Auditory Sensory Organs Ksenia Gnedeva1,2, A. J. Hudspeth3, Neil Segil1,2 1Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine of the University of Southern California, 2Caruso Department of Otolaryngology-Head and Neck Surgery, Keck School of Medicine of the University of Southern California, 3Howard Hughes Medical Institute and Laboratory of Sensory Neuroscience, The Rockefeller University Three-dimensional organotypic cultures of the murine utricle and cochlea in optically clear collagen I gels preserve innate tissue morphology, allow for mechanical stimulation through adjustment of matrix stiffness, and permit virus-mediated gene delivery.
Isolation of Myofibroblasts from Mouse and Human Esophagus Matthew Gargus1, Chao Niu1, Anisa Shaker1 1Department of Medicine, Keck School of Medicine, University of Southern California We present a protocol to generate primary cultures of murine and human esophageal stromal cells with a myofibroblast phenotype. Cultured cells have spindle shaped morphology, express α-SMA and vimentin, and lack epithelial, hematopoietic and endothelial cell surface markers. Characterized stromal cells can be used in functional studies of epithelial-stromal interactions.