3 articles published in JoVE
Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells Yen-Li Wang1,2, Adrienne Hong3, Tzung-Hai Yen1,4,5,6, Hsiang-Hsi Hong1,7,8 1Chang Gung University, 2Department of Periodontics, Chang Gung Memorial Hospital, 3California Northstate University College of Medicine, 4Department of Nephrology, Clinical Poison Center, Chang Gung Memorial Hospital, 5Kidney Research Center, Chang Gung Memorial Hospital, 6Center for Tissue Engineering, Chang Gung Memorial Hospital, 7Department of Periodontics, Chang Gung Memorial Hospital, 8College of Oral Medicine, Taipei Medical University We present a protocol to investigate the mRNA expression biomarkers of periosteum-derived cells (PDCs) induced by vitamin C (vitamin C) and 1,25-dihydroxy vitamin D [1,25-(OH)2D3]. In addition, we evaluate the ability of PDCs to differentiate into osteocytes, chondrocytes, and adipocytes.
Using Caco-2 Cells to Study Lipid Transport by the Intestine Andromeda M. Nauli1, Judy D. Whittimore2 1Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, California Northstate University, 2Department of Biomedical Sciences, James H. Quillen College of Medicine, East Tennessee State University Caco-2 cells can serve as an in vitro model to study the enterocyte transport of lipids, and lipid-soluble drugs/vitamins. The permeable membrane system separates the apical from the basolateral compartment, while the lentivirus expression system offers an effective gene overexpression method. The isolation of lipoproteins is confirmed by TEM.
Removal of Trace Elements by Cupric Oxide Nanoparticles from Uranium In Situ Recovery Bleed Water and Its Effect on Cell Viability Jodi R. Schilz1, K. J. Reddy2, Sreejayan Nair3, Thomas E. Johnson4, Ronald B. Tjalkens5, Kem P. Krueger3, Suzanne Clark6 1Division of Physical Therapy, Department of Orthopedics & Rehabilitation, University of New Mexico, 2Department of Ecosystem Science and Management, University of Wyoming, 3School of Pharmacy, University of Wyoming, 4Department of Environmental and Radiological Health Sciences, Colorado State University, 5Center for Environmental Medicine, Colorado State University, 6College of Pharmacy, California Northstate University Production bleed water (PBW) was treated with cupric oxide nanoparticles (CuO-NPs) and cellular toxicity was assessed in cultured human cells. The goal of this protocol was to integrate the native environmental sample into a cell culture format assessing the changes in toxicity due to CuO-NP treatment.