2 articles published in JoVE
Frequent Tail-tip Blood Sampling in Mice for the Assessment of Pulsatile Luteinizing Hormone Secretion Richard B. McCosh1, Michael J. Kreisman1, Kellie M. Breen1 1Department of Reproductive Medicine, University of California, San Diego School of Medicine Here we present a tail-tip blood sampling protocol for frequent sample collection in unrestrained mice. This method is useful for assessing patterns of pulsatile luteinizing hormone secretion and could be adapted for analysis of other circulating factors.
A Murine Model of Group B Streptococcus Vaginal Colonization Kathryn A. Patras1, Kelly S. Doran1,2 1Department of Pediatrics, Division of Host-Microbe Systems & Therapeutics, University of California San Diego School of Medicine, 2Department of Biology and Center for Microbial Sciences, San Diego State University The purpose of this protocol is to imitate human group B Streptococcus (GBS) vaginal colonization in a murine model. This method may be used to investigate host immune responses and bacterial factors contributing to GBS vaginal persistence, as well as to test therapeutic strategies.