2 articles published in JoVE
Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay Courtney M. Anderson1, Annelies Laeremans1, Xiao-Ming Mindy Wang1, Xingyong Wu1, Bingqing Zhang1, Emerald Doolittle1, Jeffrey Kim1, Na Li1, Helly Xiao Yan Pimentel1, Emily Park1, Xiao-Jun Ma1 1Advanced Cell Diagnostics, Inc Here, we describe an in situ hybridization assay which enables sensitive and specific detection of sequences as short as 50 nucleotides with single-nucleotide resolution at the single-cell level. The assay, which can be performed manually or automatically, can enable visualization of splice variants, short sequences, and mutations within the tissue context.
RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma Hongwei Wang1, Mindy Xiao-Ming Wang1, Nan Su1, Li-chong Wang1, Xingyong Wu1, Son Bui1, Allissa Nielsen1, Hong-Thuy Vo1, Nina Nguyen1, Yuling Luo1, Xiao-Jun Ma1 1Advanced Cell Diagnostics, Inc. The presence of high-risk HPV in head and neck tumor tissue is associated with favorable outcomes. The recently developed RNA in situ hybridization technique called RNAscope allows direct visualization of HPV E6/E7 mRNA in FFPE tissue sections.