VA Medical Center and UCSF 1 article published in JoVE Medicine Pre-clinical Orthotopic Murine Model of Human Prostate Cancer Varahram Shahryari1, Hannah Nip1, Sharanjot Saini1, Altaf A. Dar2, Soichiro Yamamura1, Yozo Mitsui1, Melissa Colden1, Nathan Bucay1, Laura Z. Tabatabai1, Kirsten Greene1, Guoren Deng1, Yuichiro Tanaka1, Rajvir Dahiya1, Shahana Majid1 1Department of Urology, VA Medical Center and UCSF, 2California Pacific Medical Center Research Institute Prostate cancer is the second most common cause of cancer-related deaths in the United States. An orthotopic cancer model provides a useful approach to understand the biology of prostate cancer and to evaluate the efficacy of therapeutic regimens. This protocol describes detailed steps necessary to establish an orthotopic prostate cancer mouse model.
