3 articles published in JoVE
Elevated Plus Maze Test Combined with Video Tracking Software to Investigate the Anxiolytic Effect of Exogenous Ketogenic Supplements Csilla Ari1, Dominic P. D’Agostino2,3, David M. Diamond1,2, Mark Kindy4,5,6, Collin Park1, Zsolt Kovács7 1Department of Psychology, Hyperbaric Neuroscience Research Laboratory, University of South Florida, 2Department of Molecular Pharmacology and Physiology, Metabolic Medicine Research Laboratory, Morsani College of Medicine, University of South Florida, 3Institute for Human and Machine Cognition, 4Department of Pharmaceutical Sciences, College of Pharmacy, University of South Florida, 5James A. Haley VA Medical Center, 6Shriners Hospital for Children, 7Savaria Department of Biology, ELTE Eötvös Loránd University Here, we present a protocol to investigate changes in the anxiety level of rodent animal models. The elevated plus maze (EPM) test, used together with a video tracking software, provides a reliable method to document the effect of various potential anxiolytic treatments in preclinical laboratory scenarios.
Calibrated Forceps Model of Spinal Cord Compression Injury Ashley McDonough*1,2, Angela Monterrubio*1,2, Jeanelle Ariza1,2, Verónica Martínez-Cerdeño1,2 1Department of Pathology and Laboratory Medicine, University of California, Davis, 2Institute for Pediatric Regenerative Medicine, Shriners Hospitals for Children (Northern California) Spinal cord injury models should be highly reproducible. We demonstrate that the calibrated forceps compression model of spinal cord injury is an easy to use surgical method for generating reproducible injuries to the murine spinal cord.
A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders Dennis J. Wu1, Neha Dixit1, Erika Suzuki1, Thanh Nguyen2, Hyun Seock Shin1, Jack Davis2, Emanual Maverakis3, Iannis E. Adamopoulos1,2 1Division of Rheumatology, Allergy and Clinical Immunology, University of California, Davis, 2Institute for Pediatric Regenerative Medicine, Shriners Hospitals for Children - Northern California, 3Department of Dermatology, University of California, Davis Differentiation of precursor cells into osteoclasts is regulated by cytokines and growth factors. Here, a novel gene transfer technique for differentiation of osteoclasts in vivo and cell culture protocols for differentiating precursor cells into osteoclasts in vitro as a method to study the effects of cytokines on osteoclastogenesis are described.